Embryo Transfer - The Horse Forum

Reply
 
LinkBack Thread Tools
post #1 of 15 Old 07-20-2011, 11:23 PM Thread Starter
Foal
 
Join Date: Jun 2011
Location: Australia
Posts: 142
• Horses: 3
Embryo Transfer

A friend and I were talking last night about embryo transfer (a topic that neither of us know much about) and a few questions came up.

Firstly, we were curious as to the process involved in 'harvesting' (correct term??) a mares eggs.
How many do the get at one time?
How they collect them?
How do they store them?
How long they can be stored?

Also, when they fertilise the egg, I assume they do it in a petrie dish and later insert the fertilised egg in the mare - much the same as they would with a human... Is this correct? If not, how do they do it?

How do people choose a surrogate mare? Is is simply a matter of convenience, or is there a lot more thought involved (ie, mares conformation, size, previous breeding history, temprement etc) (I'm assuming its the second one, but you never know!)

I find the whole subject very interesting, so I hope no one minds me asking my questions here...

Thanks in advance!
OTTBLover is offline  
Sponsored Links
Advertisement
 
post #2 of 15 Old 07-20-2011, 11:31 PM
Banned
 
Join Date: Jan 2007
Posts: 4,648
• Horses: 6
Firstly, we were curious as to the process involved in 'harvesting' (correct term??) a mares eggs.
I'm no expert either, but I've assisted in several, so let's see what I can answer...
How many do the get at one time? just one
How they collect them? you impregnate the mare with the usual process (generally AI, probably with hormone cycling to time it perfectly), then "flush" the embryo
How do they store them? in a Petri dish, as you correctly guessed
How long they can be stored? I believe it's generally just short-term, to check for grade and viability, and then they are implanted in the recipient mare soon after


How do people choose a surrogate mare? Is is simply a matter of convenience, or is there a lot more thought involved (ie, mares conformation, size, previous breeding history, temprement etc) (I'm assuming its the second one, but you never know!) well, you wouldn't want an itty bitty mare or one with wonky hips, but other than that, I've never seen conformation or size be a concern; she must be at least halter broke and easy to work around, but that's about it--and I've seen them use both maiden mares and proven mommas
bubba13 is offline  
post #3 of 15 Old 07-20-2011, 11:51 PM
Foal
 
Join Date: May 2011
Location: Wynnewood, Oklahoma
Posts: 86
• Horses: 0
Quote:
Originally Posted by OTTBLover View Post

Firstly, we were curious as to the process involved in 'harvesting' (correct term??) a mares eggs.
In the equine, harvesting a mare's eggs is a surgical procedure. I think you are looking at harvesting the embryo, or flushing the embryo is more commonly the term used.

Quote:
How many do the get at one time?
The equine is difficult to "super ovulate". Most other mammalian species can be hormonally manipulated to produce multiple embryos. With the equine, even using eFSH (equine follicle stimulating hormone) it only doubles your chances. The industry average for flushing embryos is 50% - so 50% of the time, you will flush an embryo...with the use of eFSH, you double your chances to, drum roll please...1.

Quote:
How they collect them?
The mare's uterus is filled with a flush media that is specifically formulated for doing embryo transfers. The flush is then run back through a catheter that has a filter attached that will capture the embryo. The fluid that is in the filter is then viewed under a microscope and the search begins :).

Quote:
How do they store them?
In most cases, the embryo is immediately transferred into a recipient mare. However, there is now a procedure where the embryo can be frozen and stored. Success rate of transferring the thawed embryo into a recip mare is comparable to those of embryos that are transferred immediately.

Quote:
How long they can be stored?
It is speculated that they can be stored for as long as frozen semen - 40,000 years.

Quote:
Also, when they fertilise the egg, I assume they do it in a petrie dish and later insert the fertilised egg in the mare - much the same as they would with a human... Is this correct? If not, how do they do it?
No. Invitro fertilization is not done in the equine - at least not thesame way it is done with other mammals. If one does harvest a mare's eggs, in order to fertilize the egg, ICSI (Intracytoplasmic Injection) must be used where a single sperm is actually injected directly into the ovum.

Quote:
How do people choose a surrogate mare? Is is simply a matter of convenience, or is there a lot more thought involved (ie, mares conformation, size, previous breeding history, temprement etc) (I'm assuming its the second one, but you never know!)
It is preferred that a mare of comparable size is used, that is under the age of ten and older than four, that has had at least one foal and is reproductively sound. Obviously, you want a mare with a known temperament that is good for nuturing the foal, as well as human friendly.

Hope the above helps!

Last edited by Avalon Equine; 07-21-2011 at 12:00 AM.
Avalon Equine is offline  
post #4 of 15 Old 07-21-2011, 12:41 AM Thread Starter
Foal
 
Join Date: Jun 2011
Location: Australia
Posts: 142
• Horses: 3
Avalon Equine - Thankyou for taking the time to give such a comprehensive and informative answer!
I learned a lot

It is certainly more involved than what I imagined!
OTTBLover is offline  
post #5 of 15 Old 07-21-2011, 12:46 AM
Yearling
 
Join Date: Jul 2008
Location: Riverside, CA
Posts: 971
• Horses: 4
There is a ranch near me that does embryo transfers. They use QH and TB mares.

Dana
Riverside, CA
danastark is offline  
post #6 of 15 Old 07-21-2011, 02:05 AM
Foal
 
Join Date: Feb 2011
Posts: 246
• Horses: 0
This is a good thread, im thinking about doing an embryo transfer for one of my mares this breeding season!
wild horses is offline  
post #7 of 15 Old 07-21-2011, 04:03 AM Thread Starter
Foal
 
Join Date: Jun 2011
Location: Australia
Posts: 142
• Horses: 3
Quote:
Originally Posted by wild horses View Post
This is a good thread, im thinking about doing an embryo transfer for one of my mares this breeding season!
That leads me into my next question - what are the reasons someone would do an embryo transfer?
OTTBLover is offline  
post #8 of 15 Old 07-21-2011, 11:23 AM
Trained
 
Join Date: Jan 2009
Location: N.W. Ohio
Posts: 5,344
• Horses: 0
When I do it with my mares it is b/c I have a buyer for the embryo. Works out well for all involved. They get a better price and I still get to use my mare.

-I'm so busy... I don't know if I found a rope or lost my horse.
-An Armed Man is a Citizen an unarmed man is a subject.
-Where ever free speech is stifled Tyranny will reign.
nrhareiner is offline  
post #9 of 15 Old 07-21-2011, 01:56 PM
Weanling
 
Join Date: Dec 2008
Posts: 455
• Horses: 0
What is the average cost tied up in this procedure?
SaddleUp158 is offline  
post #10 of 15 Old 07-21-2011, 02:17 PM
Green Broke
 
Join Date: Dec 2009
Posts: 3,968
• Horses: 8
I know with some breeds at least, it is often done to get more than one foal out of a certain mare in one year. Breed mare, flush embryo, and then breed mare again nest heat for a pregnancy herself.

I read a study a few years back on a place (I think it was the University of CO) that was using cycling mules (true mollies) as surrogates - fascinating stuff, actually, because although mules are infertile hybrids, they have all the working parts, and can carry, deliver, nurse and raise an implanted foal very well and tend to make VERY good moms.


Indyhorse is offline  
Reply

Quick Reply
Message:
Options

Register Now



In order to be able to post messages on the The Horse Forum forums, you must first register.

Already have a Horse Forum account?
Members are allowed only one account per person at the Horse Forum, so if you've made an account here in the past you'll need to continue using that account. Please do not create a new account or you may lose access to the Horse Forum. If you need help recovering your existing account, please Contact Us. We'll be glad to help!

New to the Horse Forum?
Please choose a username you will be satisfied with using for the duration of your membership at the Horse Forum. We do not change members' usernames upon request because that would make it difficult for everyone to keep track of who is who on the forum. For that reason, please do not incorporate your horse's name into your username so that you are not stuck with a username related to a horse you may no longer have some day, or use any other username you may no longer identify with or care for in the future.



User Name:
Password
Please enter a password for your user account. Note that passwords are case-sensitive.

Password:


Confirm Password:
Email Address
Please enter a valid email address for yourself.

Email Address:
OR

Log-in









Human Verification

In order to verify that you are a human and not a spam bot, please enter the answer into the following box below based on the instructions contained in the graphic.


Old Thread Warning
This thread is more than 90 days old. When a thread is this old, it is often better to start a new thread rather than post to it. However, If you feel you have something of value to add to this particular thread, you can do so by checking the box below before submitting your post.

Thread Tools
Show Printable Version Show Printable Version
Email this Page Email this Page



Similar Threads
Thread Thread Starter Forum Replies Last Post
Embryo from GP Successful Jumper Mare SunShineMeadows Horses for Sale 1 05-07-2011 03:12 PM
Transfer of Registration Remi Horse Talk 7 10-18-2010 05:24 AM
Registration Paper Transfer Horsegma Horse Talk 14 01-11-2010 06:29 PM
Help filling out transfer papers myQHpaul Horse Talk 6 06-30-2009 02:37 AM
APHA transfer papers SonnyWimps Horse Talk 2 03-13-2008 09:12 AM

Posting Rules  
You may post new threads
You may post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

 
For the best viewing experience please update your browser to Google Chrome