Why the Estromate shot? That is not needed. Put her on Regu mate for 15 days pull her off I would work it so she is coming off on a Mon or Tues but that is just me. She will come into season about 3 to 4 days latter and should be ready to breed on day 5. I have been doing it this way for years with very good success I do not give any extra shots. They are not needed 98% of the time. Also make sure she is past her transitional heat or you are just wasting your money.
If you thaught she was coming in few days ago and then nothing that is quite common this time of year around here and she is not trainsitional yet.
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