That's an interesting question. I know only overos will have blue eyes, but which gene causes them? I was trying to read about it just now and it only mentions blue eyes in the overo category: Overo
: A group of spotting patterns characterized by sharp, irregular markings with a horizontal orientation, usually more dark than white, though the face is usually white, sometimes with blue eyes
. The white rarely crosses the back, and the lower legs are normally dark. The APHA recognizes three overo patterns:
And then in the spalsh sub-category: Splashed white
: The least common spotting pattern, splashed whites typically have blue eyes
and crisp, smooth, blocky white markings that almost always include the head and legs. The tail is often white or white-tipped, and body markings originate under the belly and extend "upwards".
It doesn't mention anything about blue eyes in frame or sabino.
So educate me weefoal LOL