Edited beacsue I didn't know this was an old thread. Glad she was tested!
Splash causes blue eyes, frame does not. There is a great chance she is both. I know solid horses that carry fram if its in her pedigree at all I would get her tested if you're ever thinking of breeding her.
So far it seems pretty certain that Sabino doesn't cause blue eyes. So splash is the cause :)
I think Sabino in homozygous form may influence blue eyes...then again a lot of what we think is Sabino we are finding out is actually dominant white, so it could be dominant white does and sabino doesn't...mysteries of horse colors...