I think it's ok to call. I would introduce myself, indicate I had contacted the facility via email/letter a few days prior, and was calling to follow up on my concerns. If you sent a snail-mail letter, them probably hold off calling until its reasonable they received the letter.
Since it seems like you know the name of the business owner, I would firmly (but politely) insist to speak with her. If you get the "she's not available" line from staff, then ask to be forwarded to her voicemail, and for advice on what time she is typically near her phone so you can continue to follow up.
I too am curious to hear how this turns out! If you're ever in Vermont, I can recommend a gorgeous trail facility with beautiful mountain views and wide open grass-and-wildflower pastures to canter through! :)
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